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We have mentioned earlier that radio-opaque granules migrate from oocyte cytoplasm to its periphery, which ultimately give rise to a glycoprotein layer called Zona-pellucida.             This study has demonstrated the role of Golgi Apparatus, secretory granules and vesicular aggregates in mouse oocyte in Zona-pellucida synthesis. Barcode Creator Software Barcode Studio 11 0 0 - The mechanism of matrix formation and stoichiometric disposition of ZP1, ZP2, & ZP3 in mouse oocyte development has been investigated (Mestrah et al, 2002). Barcode Creator Software Barcode Studio 11 0 0 - A new technique using antibodies against on intracellular material trafficking from oocyte for synthesize Zona-pellucida. Department of Anatomy, cell Biology laboratory of health science, Queen's University Canada has investigated the subcellular distribution of ZPI zona-pellucida 1,2 and 3 glycoproteins during folliculogenesis (Mestrah et al, 2002). Barcode Creator Software Barcode Studio 11 0 0 -             The actions of insulin like growth factors (IGF-I) on rabbit ovarian cells has been investigate4d. Barcode Creator Software Barcode Studio 11 0 0 - Zona pellucida (ZP)   is an extra cellular coat synthesized by oocyte during development.